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Fluorescence and Luminescence Spectroscopy:

The NDE system is a Perkin-Elmer LS-50B with WinLabT software. The system can perform routine analysis, custom analysis, and method development tasks. The wide range of UV and visible wavelengths allows the detection of essentially all the fluorescent dyes used in biological assays. This capability is useful for the following measurements:

• Fluorescence, phosphorescence, bioluminescece, and chemiluminescence of a variety of samples.

• Determination of excitation and emission wavelengths for unknown fluorescence sources.

• 3D excitation/emission scans, 3D synchronous and kinetic scans.

• Microplate measurements with fixed wavelength, programmable wavelength, or automated spectral data collection.

• Accurate quantification using different curve fitting algorithms.

Added software features include a GLP validation protocol. A plate reader accessory for the measurement of fluorescence-based assays in multi-well plates is also available.